ATAR Notes: Forum
HSC Stuff => HSC Science Stuff => HSC Subjects + Help => HSC Biology => Topic started by: I.09 on April 27, 2021, 08:10:15 pm
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Hey Guys,
I have been stuck on this for ages..
so when the biotechnology of CRISPR is used to cut the double-stranded DNA of the cell and a new gene is inserted, where does this new gene come from? Does it come from another organism or is it synthesised by humans?
I am so confused, any help will be greatly appreciated.
Thank you so much.
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A new gene can come from insertion of a template gene via the homology directed repair pathway, or random bases fill in where the Cas protein cuts via the Non-Homolgous End Joining pathway. (The former is called Knock-in, and the latter often causes a gene knock-out)
These pathways are competitive against each other, and NHEJ is more commonly observed compared to HDR.
Furthermore, there are several other ways to insert a gene or change a base with CRISPR/Cas9 such as base editing and prime base editing but that may be out of your syllabus scope :)
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Thank you so much!!
So, those are the two ways in which genes can be inserted via CRISPR, but is the gene itself from another organism or lab-made?
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Thank you so much!!
So, those are the two ways in which genes can be inserted via CRISPR, but is the gene itself from another organism or lab-made?
It can be from the organism itself (for bases) or as an external template (I.e. lab-made or from other gene sources)