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May 19, 2024, 02:52:45 am

Author Topic: Bacterial Transformation  (Read 1768 times)  Share 

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shahifa

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Bacterial Transformation
« on: September 07, 2021, 08:40:29 pm »
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Hi guys

could someone explain to me how PCR and Gel electrophoresis is used when leading up to bacterial transformation?

stickyends

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Re: Bacterial Transformation
« Reply #1 on: September 09, 2021, 10:25:37 pm »
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Hi!
If a particular gene to be transformed is isolated from an organism e.g. a human, then PCR can be used firstly to amplify that sample of DNA and make many copies of the gene. Then, gel electrophoresis is used along with a standard ladder to isolate the gene of interest. Remember, gel electrophoresis requires millions of copies of DNA in order for it to be visible. After doing this, the isolated gene of interest can be inserted into the plasmid and used in bacterial transformation.
Hope this helps!!

shahifa

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Re: Bacterial Transformation
« Reply #2 on: September 10, 2021, 12:52:57 pm »
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Ahh yes that makes sense! thank you!!