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VCE Stuff => VCE Science => VCE Mathematics/Science/Technology => VCE Subjects + Help => VCE Chemistry => Topic started by: macca69831 on March 13, 2010, 11:21:44 am

Title: HPLC and GLC
Post by: macca69831 on March 13, 2010, 11:21:44 am

1) State three similarities between the design and function of HPLC and GLC

2) State two differences between the design and function of HPLC and GLC

Title: Re: HPLC and GLC
Post by: monokekie on March 13, 2010, 11:29:17 am

1) stationary phase+mobile phase, detector & recorder, sample injection port, can be used to find the Rt value of different sample components.

2) HPLC uses liquid/aqueous mobile phase while GLC uses Gaseous(inert, usually nitrogen) mobile phase. HPLC can be performed under normal laboratory temperatures where as GLC has to be performed within an oven to ensure the sample are vaporised. Only samples that have low boiling point can be analysed using GLC as they need to be in a gaseous form to mix with carrier gas.

Title: Re: HPLC and GLC
Post by: physics on March 13, 2010, 09:22:41 pm

detector ,recorder and retention time

HPLC=high pressures and porous solid coated wiuth vicious liquid
GLC= gas and oven