ATAR Notes: Forum

VCE Stuff => VCE Science => VCE Mathematics/Science/Technology => VCE Subjects + Help => VCE Biology => Topic started by: itsMRPHI on October 28, 2010, 08:09:39 pm

Title: PCR
Post by: itsMRPHI on October 28, 2010, 08:09:39 pm

Do nucleotides get added from the 5' to 3' direction or the 3' to 5' direction?

Title: Re: PCR
Post by: jasoN- on October 28, 2010, 08:10:34 pm

3' to 5'
page 3 http://www.vcaa.vic.edu.au/vce/studies/biology/asessreports/2007/biology2_assessrep_07.pdf

Title: Re: PCR
Post by: Russ on October 28, 2010, 08:12:32 pm

Erm, what?

Title: Re: PCR
Post by: TrueLight on October 28, 2010, 08:17:22 pm

Quote from: Russ on October 28, 2010, 08:12:32 pm

Erm, what?

Title: Re: PCR
Post by: shinny on October 28, 2010, 08:25:56 pm

The enzymes read/act in a 3' to 5' direction, meaning the nucleotides are added in the 5' to 3' direction with respect to the complementary strand.

Title: Re: PCR
Post by: masonnnn on October 28, 2010, 08:56:11 pm

Quote from: shinny on October 28, 2010, 08:25:56 pm

The enzymes read/act in a 3' to 5' direction, meaning the nucleotides are added in the 5' to 3' direction with respect to the complementary strand.

This.
Just know that no matter what they are always ADDED to the 3' end.
this gets confusing as because they are added to the 3' end the template is actually read in a 3'-->5' direction.

Title: Re: PCR
Post by: itsMRPHI on October 28, 2010, 10:36:23 pm

cheers mason