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November 01, 2025, 12:09:37 pm
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Author Topic: Stationary phase-Chromotography  (Read 872 times)  Share 

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chris19021

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Stationary phase-Chromotography
« on: March 12, 2012, 07:50:30 pm »
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Hai wht is the purpose of the stationary phase in chromotography?
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eaa

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Re: Stationary phase-Chromotography
« Reply #1 on: March 13, 2012, 10:01:14 am »
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The stationary phase is where the mobile phase passes through and the distribution of the sample between the phases occur.
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Hutchoo

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Re: Stationary phase-Chromotography
« Reply #2 on: March 13, 2012, 06:22:20 pm »
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Essentially, the stationary phase lets substances 'break down' into their individual components. It's useful because it lets us determine which substances adsorb more readily to the stationary phase, thus producing either a retention time or retention factor (depending on the type of chromatography analysis).
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Shenz0r

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Re: Stationary phase-Chromotography
« Reply #3 on: March 13, 2012, 06:33:52 pm »
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The components separate due to differing levels of adsorption (the temporary binding of a component to the stationary phase), and thus components have different retention times.

The mobile phase kind of "sweeps" the components down the stationary phase.
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Hutchoo

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Re: Stationary phase-Chromotography
« Reply #4 on: March 13, 2012, 07:05:35 pm »
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Quote from: Shenz0r on March 13, 2012, 06:33:52 pm
The components separate due to differing levels of adsorption (the temporary binding of a component to the stationary phase), and thus components have different retention times.

The mobile phase kind of "sweeps" the components down the stationary phase.
Not all chromatography have retention times (paper chromatography for example).
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