Questions

[Dark Horse]

Hey guys, just a few questions that I have before midyears

Does the sample size you inject into a GC or a HPLC affect the resolution of components?

also, `i always lose marks in enzyme questions because I forget "key words", bcause I say that the enzyme is denatured. What do I have to mention about the tertiary and secondary structure in these questions?

And what should we know about protein markers? Should we memorise one or more?

Thanks

[TrueTears]

Hey guys, just a few questions that I have before midyears

Does the sample size you inject into a GC or a HPLC affect the resolution of components?

also, `i always lose marks in enzyme questions because I forget "key words", bcause I say that the enzyme is denatured. What do I have to mention about the tertiary and secondary structure in these questions?

And what should we know about protein markers? Should we memorise one or more?

Thanks

The size of the components may affect the retention time. If you have a very large component it may take longer to get through the HPLC column since the stationary phase may "block" it from passing, hence higher retention time.

What is the exact type of enzyme questions you are not sure about? Basically if it is about primary, secondary or tertiary structure, you should know all the different kinds of bonding that occurs.

You are not required to know any exact protein markers, but it'd be a good idea to know one just in case. For example, a rise in the level of a certain enzyme when heart attacks occur.

[NE2000]

Hey guys, just a few questions that I have before midyears

Does the sample size you inject into a GC or a HPLC affect the resolution of components?

also, `i always lose marks in enzyme questions because I forget "key words", bcause I say that the enzyme is denatured. What do I have to mention about the tertiary and secondary structure in these questions?

And what should we know about protein markers? Should we memorise one or more?

Thanks

The way GC and HPLC work means that you may get some overlap between peaks if you inject too many components in the sample at once. This is particularly true for components of similar size (eg. a similarly sized alkene and alkane inserted together). It's a problem with all types of chromatography and to overcome this you would try varying the conditions or lengthening the column to achieve better resolution.

With enzymes, denaturation of an enzyme is the disruption of its tertiary and secondary structures. This alters its shape such that it is no longer able to complete its initial function. I think that should do it. More info could be that high temperatures break the intermolecular forces that hold the tertiary structures togther.

Just know that proteins markers are specific unique proteins that are released by diseased or damaged organs and can be monitored to test for the development of a disease. Memorizing one out of your book should serve you just fine.