DNA fingerprinting question

[Jarzoon]

Just a few questions here..

In the method of DNA fingerprinting, does the DNA amplification prcoess using PCR step come before cutting the DNA using restrictive enzymes? or is it the other way round? or can it be both?

Also, do immersion in radioactive probes have to be after the separation gel electrophoresis process?

Thanks

[d0minicz]

DNA is amplified first using PCR, then cut into fragments by restriction enzymes => then to electrophoresis

[Jarzoon]

okay thanks
it's just that i've seen them in different orders in some of the textbooks and solutions to questions...

[mystikal]

dont forget that it has to be shone in UV light to see where the cuts are

[linny]

1. restriction enzymes break DNA into fragments
2. separation by electrophresis
3. immersion in radioactive probes

so yeah, immersion in radioactive probes have to be after electrophresis

[arthurk]

restriction enzymes what about the PCR there linny

[linny]

lol im answering his second question there arthurk

"Also, do immersion in radioactive probes have to be after the separation gel electrophoresis process?"