Does temperature affect HPLC?

[ckboz]

HI, i am studying yr 12 chemistry, and i just want to find out whether decreasing the temperature of the sample will causes it to have a shorter retention time or not.
I think that if you have a sample with lower temperature, the sample won't expand, therefore, they will be able to travel through the gaps quicker and faster and resulting in a shorter retention time.
and also, with lower temperature, the kinetic energy that the particle has will be less and therefore, there will be less adsorption and desorption which will also result in a shorter retention time, but i am not sure if my ideas are correct or not.
thanks for reading

[Yacoubb]

HI, i am studying yr 12 chemistry, and i just want to find out whether decreasing the temperature of the sample will causes it to have a shorter retention time or not.
I think that if you have a sample with lower temperature, the sample won't expand, therefore, they will be able to travel through the gaps quicker and faster and resulting in a shorter retention time.
and also, with lower temperature, the kinetic energy that the particle has will be less and therefore, there will be less adsorption and desorption which will also result in a shorter retention time, but i am not sure if my ideas are correct or not.
thanks for reading

Retention time is the time taken for a component to move through the column. If we decrease the temperature of the sample, the sample moves through the column much slower and thus takes more time to move through the column. Hence, the retention time is longer if the temperature is decreased. Conversely, increasing the temperature results in the movement of the components through the column at an increased rate. The components then move through the column faster, and thus spend less time in the column. Hence, the retention time is shorter at higher temperatures.