Questions

[TrebleClef]

How do you know where exactly the bonds occur between complementary nitrogenous bases?
Thank you!

[Reus]

Just know that the bonds occurring between complementary base pairs (AT & GC) are formed by hydrogen bonding (H-FON) and that Guanine and Cytosine have 3 Hydrogen bonds between them whereas Adenine and Thymine only have 2.

So just bind them between H-N or O-H etc...
For example Adenine's NH2 is to Thymine's O (double bonded to C) and N to HN.

[TrebleClef]

Just know that the bonds occurring between complementary base pairs (AT & GC) are formed by hydrogen bonding (H-FON) and that Guanine and Cytosine have 3 Hydrogen bonds between them whereas Adenine and Thymine only have 2.

So just bind them between H-N or O-H etc...
For example Adenine's NH2 is to Thymine's O (double bonded to C) and N to HN.

thank you!

Also, can someone explain to me what exactly the base peak represents, and how would we out what the base peak is if we were given a formula. Thanks!

[Reus]

thank you!

Also, can someone explain to me what exactly the base peak represents, and how would we out what the base peak is if we were given a formula. Thanks!

Well in a mass spectrum the data is given in a vertical bar graph, where the bars represent an ion having a specific mass to charge ratio (m/z) and the length of the bar indicates the relative abundance of the ion (relative concentration of ions present).

The base peak represents the most intense ion which is assigned an abundance of 100% (longest bar/peak). In some cases, the parent molecular ion is the base peak.
To work this out, you can use the method of fragmentation.

[TrebleClef]

Well in a mass spectrum the data is given in a vertical bar graph, where the bars represent an ion having a specific mass to charge ratio (m/z) and the length of the bar indicates the relative abundance of the ion (relative concentration of ions present).

The base peak represents the most intense ion which is assigned an abundance of 100% (longest bar/peak). In some cases, the parent molecular ion is the base peak.
To work this out, you can use the method of fragmentation.

thank you!

[Jnf17]

How do you know where exactly the bonds occur between complementary nitrogenous bases?
Thank you!

On the data sheet where the four bases are listed - bonding occurs going anticlockwise starting at the top atom. So for A and T bonding occurs on the first two atoms as you go anticlockwise around the base starting from the top. And for C and G same thing but the first three atoms.
Hope that helps