Uni Stuff > The University Journey Journal
A Spoonful of Sugar- insanipi's PharmSci journey
sweetiepi:
Howdy!
Just a cheeky morning update whilst I let breakfast settle before I write that monthly report I owe :-X
I honestly feel like I've done squat bar from being a slave to my cells tbh- they're needy little critters! But I have actually done a lot (including having to get up early this morning and nab the autoclave before ANYONE could use it, leaving me to have breakfast after the quick lab session haha). I just can't believe that it's nearly the end of July because oh gosh I have about 1 page written for my thesis and still no actual usuable results and I'm definitely feeling the heat D:
This week I'm mainly focusing on amplifying my DNA plasmid because I have like 2 ul of it left and kinda need more! I tried to start that process Thursday, but couldn't (because the autoclave door was jammed), and again on Friday (some jerks booked it out the whole day), so now I've started the process this morning! I've also thawed some of my GFP-expressing cells down so I can personally make some stocks sometime this week (because previously I had to bleach my batch because we had no cryovials at the time :( )
This week will probably be a chill week otherwise in the lab (pending my weekly discussion with Tom-the-PhD-pal), which is good because I kinda need the spare time as this report won't write itself (and I need to make a presentation for the big group meeting on Friday too and I currently have nfi to put in that either meeeeeeeeeeeeeeeeeeeeeep haha)
Love y'all <3
sweetiepi:
Howdy!
I'm still allowed to come to uni to do lab work, so that's hella nifty! I got my first official bits of data last week and I'm doing my first proper experiment of the project this week- but that's already hit a bit of a bump as I have found that I can't just go do a quick check on the plate reader to see if it works as the blue led sucks, so it'll be either Wednesday or Thursday when I find out if it works or not when I go use flow cytometery 🙈
Aside from that, I was well rested over the weekend, but I just seeded (or plated) 4 plates worth of cells and I'm sapped. I still have 2.5h to go because I'm also making an LNP formulation today. Then I'm here from 8:15 until 5:30 tomorrow, because I'm a slave to my cells haha.
Anywho that's all from me, stay safe xo
sweetiepi:
THE BASIS OF MY WHOLE PROJECT ACTUALLY WORKS! 😍
I ran flow cytometery on Friday for measuring the fluorescence of my cells and I got around 2% positive! This isn't much, but we know it works- so all my hard work so far has been worth it!
Want to know what a typical day of mine looks like at the moment?
Let's use today for an example!
6:00am: wake up
6:40: brekky
6:50: leave house for train
7:07: train
8:05: arrive at uni
8:10-8:30: cell culture- coating plates with poly-d-lysine and checking on a plate from last week.
8:30-9:00: coffee/tea and analysis break.
9:00: retrieves cell culture media from other lab to thaw and make up some new stock because I was lazy the previous week and now desperately need to make more
9:30-10:30: cell culture- moving some experimental cultured plates to an 8 well slide for microscopy later today
10:30-11:00: break for data analysis
11:00-11:30: meeting with my PhD pal to discuss this week's experiments
11:30-12:00: outside break
12:00-1:00: microscopy
1:00-2:00: lunch and planning for the week's experiments
2:00-3:30: cell culture- moving my lipopeptide plate to flow cytometry tubes
3:30-4:30: flow cytometery
4:30-6:00: get home
Stay home/stay safe y'all ❤
Joseph41:
Great news! Well done - happy for you. :)
homeworkisapotato:
So happy for you <33
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