Uni Stuff > The University Journey Journal
A Spoonful of Sugar- insanipi's PharmSci journey
The Cat In The Hat:
Despite my not understanding practically anything about this stuff, it's really interesting. :)
AngelWings:
--- Quote from: insanipi on September 07, 2020, 08:52:49 am ---THE BASIS OF MY WHOLE PROJECT ACTUALLY WORKS! 😍
I ran flow cytometery on Friday for measuring the fluorescence of my cells and I got around 2% positive! This isn't much, but we know it works- so all my hard work so far has been worth it!
--- End quote ---
That’s amazing to hear! It’s not often that things actually work, especially something experimental (and harder still when it’s during a pandemic). Congrats on this milestone!
sweetiepi:
--- Quote from: Joseph41 on September 07, 2020, 08:55:57 am ---Great news! Well done - happy for you. :)
--- End quote ---
--- Quote from: homeworkisapotato on September 07, 2020, 08:57:32 am ---So happy for you <33
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💚💚💚
--- Quote from: The Cat In The Hat on September 07, 2020, 09:17:37 am ---Despite my not understanding practically anything about this stuff, it's really interesting. :)
--- End quote ---
Don't worry, I'm still getting my head around it too, despite being nearly 9 months deep into the project haha :P
--- Quote from: AngelWings on September 07, 2020, 10:32:34 am --- That’s amazing to hear! It’s not often that things actually work, especially something experimental (and harder still when it’s during a pandemic). Congrats on this milestone!
--- End quote ---
I was genuinely expecting it not to work, but alas, it worked! I still cannot get one of the delivery methods to work though (trialling with plain cells to GFP), no matter what I do- it doesn't work aha :P
I also got some cool images from the microscopy yesterday, which confirms that seeing what I did on flow cytometry last week wasn't just a fluke! :)
sweetiepi:
A bit of a quick update today! It's been nearly a month, and I haven't really acheived much else aside from getting replicates for the data I already had.
This week already has been a drainer and a half- I had a lab class Monday, and I'm in the middle of preparing for my journal critique assessment tomorrow- which is on top of me just setting (just under) half my assays I had planned- I was meant to get another two started, but a machine that I absolutely need busted itself Tuesday, so I don't know when that'll be fixed because I kinda need to get my stuff done and obviously can't (there's only one of this machine on campus, so that's a yikes and a half if I ever saw one :'))
On the bright side of things- I got some pretty cell images yesterday night, and I now have the right to keep my scrunchie obsession up haha!
That's all for now, toodles! <3
sweetiepi:
--- Quote from: insanipi on October 01, 2020, 11:00:05 am ---A bit of a quick update today! It's been nearly a month, and I haven't really acheived much else aside from getting replicates for the data I already had.
This week already has been a drainer and a half- I had a lab class Monday, and I'm in the middle of preparing for my journal critique assessment tomorrow- which is on top of me just setting (just under) half my assays I had planned- I was meant to get another two started, but a machine that I absolutely need busted itself Tuesday, so I don't know when that'll be fixed because I kinda need to get my stuff done and obviously can't (there's only one of this machine on campus, so that's a yikes and a half if I ever saw one :'))
On the bright side of things- I got some pretty cell images yesterday night, and I now have the right to keep my scrunchie obsession up haha!
That's all for now, toodles! <3
--- End quote ---
Following on from this, last week I had a terrible cell contamination event happen so I couldn't restart my experiments when the broken machine got replaced 🙈
That means that I'm having to squeeze 14 assays into the next fortnight; I hope I can do it, because I have a deadline to meet!
Hoping I don't go too insane from it! 😅
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