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November 08, 2025, 06:59:18 am

Author Topic: Chromatography  (Read 2081 times)  Share 

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mandy

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Chromatography
« on: February 07, 2010, 05:56:16 pm »
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A pure substance was suspected of being the high molecular weight anabolic steroid Stanzolol. A sample of this substance was dissolved in a suitable pure solvent and injected into a gas chromatograph. How many peaks would you expect to see in the resulting chromatogram? Justify your answer.

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fady_22

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Re: Chromatography
« Reply #1 on: February 07, 2010, 06:25:11 pm »
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You should have only one peak, because it is supposed to be a pure substance (therefore only consists of the steroid).
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vexx

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Re: Chromatography
« Reply #2 on: February 07, 2010, 06:27:25 pm »
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You should have only one peak, because it is supposed to be a pure substance (therefore only consists of the steroid).

Yeah the amount of elements in the compound is usually the amount of peaks in a chromatogram.
the no. of compounds in the mixture = the no. of peaks.
heh thanks mao.
« Last Edit: February 07, 2010, 07:18:40 pm by vexx »
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Mao

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Re: Chromatography
« Reply #3 on: February 07, 2010, 06:58:21 pm »
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You should have only one peak, because it is supposed to be a pure substance (therefore only consists of the steroid).

You just beat me.

Yeah the amount of elements in the compound is usually the amount of peaks in a chromatogram.

You meant the number of compounds in the mixture, not to be confused with elements as in elemental compounds [O2, N2, H2, etc]
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vexx

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Re: Chromatography
« Reply #4 on: February 07, 2010, 07:17:25 pm »
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You should have only one peak, because it is supposed to be a pure substance (therefore only consists of the steroid).

You just beat me.

Yeah the amount of elements in the compound is usually the amount of peaks in a chromatogram.

You meant the number of compounds in the mixture, not to be confused with elements as in elemental compounds [O2, N2, H2, etc]

oh sorry, i completely didn't think about it properly.
yep listen to mao, i'll edit my post in case i confuse anyone.
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mandy

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Re: Chromatography
« Reply #5 on: February 07, 2010, 08:37:38 pm »
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Thanks for the help guys :]
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herzy

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Re: Chromatography
« Reply #6 on: February 07, 2010, 10:19:24 pm »
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you're thinking of mass spec :)
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mandy

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Re: Chromatography
« Reply #7 on: February 07, 2010, 11:06:21 pm »
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you're thinking of mass spec :)

oh no, so is it wrong :S
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vexx

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Re: Chromatography
« Reply #8 on: February 07, 2010, 11:07:16 pm »
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you're thinking of mass spec :)

oh no, so is it wrong :S

no what we said above was right.

i think he meant my original comment as mass spec looks at each element. and chromo looks at the compounds in a mixture :)
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mandy

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Re: Chromatography
« Reply #9 on: February 07, 2010, 11:11:30 pm »
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you're thinking of mass spec :)

oh no, so is it wrong :S

no what we said above was right.

i think he meant my original comment as mass spec looks at each element. and chromo looks at the compounds in a mixture :)

Haha, ohhh okay. Thank you!
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herzy

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Re: Chromatography
« Reply #10 on: February 07, 2010, 11:26:16 pm »
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yeah lol sorry for the ambiguity. serves me right for making tired comments wtihout reading the thread properly :)
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longy1991

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Re: Chromatography
« Reply #11 on: February 07, 2010, 11:46:27 pm »
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but it is "dissolved in a suitable pure solvent", therefore you will get two peaks if it is pure, 1 for the solvent, and 1 for the steroid.
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