how does the light dependent and light independent stage in photosynthesis work? The textbooks seems confusing can someone explain it an easier way?
I'll try to explain it in a more detailed way:
Light dependent stage:
Plants contain chlorophyll in their leaves, when they are exposed to sunlight, light energy will excite the electrons in the chlorophyll and these exited electrons are what trigger photosynthesis.
Firstly, photosynthesis can be seen as a reverse of cellular respiration in which light dependent stage occurs in a slightly similar fashion as the Electron transport chain in respiration. What's different is obviously the location of light dependent stage, which is in the thylakoid membrane (grana). Embedded in these thylakoid membrane are protein channels and enzymes that are able to produce ATP. Excited electrons will release the energy that it gains from sunlight to these protein channels and that will be used to actively pump the H+ ion out of the outer space membrane to generate a concentration gradient. When we have a sufficient concentration gradient between the inner cell and outer space membrane, H+ ions will flush back into the ATP syntheses for the production of ATP ( at this stage, you can see that it is exactly like Electron transport chain). However, the H+ ions will be shuttled by the electron carrier called NADP+ ( whilst cellular reparation is NADPH). Moreover, referring back to what happened with the chlorophyll, since it has donate some of its electrons to the protein channels, it is now electron short, and immediately need some source of electron to make up for this. This is why H20 comes into need, water will be split into H+ ion and O2 due to the high electronegativity that chlorophyll proceeds. O2 will be released out of the plant cell through stomata.
Hence, what we end up after the light dependent stage is a lot of ATP, NADPH and O2 (O2 is a by-product).
Light-indenpendent stage (Calvin cycle):
Now that we have produced a lot of ATP and NADPH, let's make some glucose. Cells use the energy from ATP to combine the H+ ion that NADPH brings and CO2 into glucose. This process in called Carbon fixation in which we are changing Inorganic carbon into Organic Carbon. Notice that we adding simple structure molecules into a complex structure, this is called anabolism ( hence it is endergonic and also a condensation process). What we get after joining molecules together? H20. This is when the RHS H20 in the equation comes from. It is worth notice that the LHS H20 and RHS H20 are not the same because one is used and one is newly formed.
Hence, what we end up after the Calvin Cycle is Glucose ( C6H12O6), NADP+, ADP + Pi, H20.
Hope this helps!