Thanks IThinkIFailed and Erutepa for the help!
From the 2017 exam, question 9 c ii, I was wondering why they said that the transformed bacteria wouldn't have ampilicillin resistance? Since the restriction enzyme used was BamH1, this means that the gene for tetracycline resistance was disrupted meaning that the transformed bacteria wouldn't have died if placed on tetracycline agar. Would this be correct?
From the same exam and question 9 b ii, I was wondering why VCAA didn't add the BamH1 recognition site on the plasmid even though the question specifically asked to do that?
Also, when talking about complement proteins, what should we talk about?
I know that we need to talk about them forming membrane attack complexes and lysing but I was wondering what other stuff we needed to know about their function and how they help?
1. Question 9 has come up before and some nice answers to it can be found here
https://atarnotes.com/forum/index.php?topic=184382.0But basically, ampicilin resistance is carried on the plasmid as the ampicilin gene is intact in the plasmid. As such, bacteria sucsessfully transformed will have ampicilin resistance, while those not sucsessfully transformed will not have ampicilin resistance and will die. This is what the examiners report says.
You seem to be getting confused between ampicilin gene and the tetracycline gene.
2. For the diagram in the examiners report, I think they just left out the label. The question asked you to label it so you should.
3. I would say that the main 3 functions are to:
Attract other immune cells function as chemoattractants.
Lyse the cell by forming a membrane attack complex
Enhance phagocytosis by binding to pathogens and acting as an opsonin.
Hey,
does DNA hybridisation require temperatures to separate DNA strands (95 and 55) and allow them to join back together, like PCR?
I usually write the temperatures in but would this be wrong
In the past they have just accepted heating and cooling. Specific temps are not needed for DNA hybridization, but are needed for PCR.