Three questions:
1) If the quaternary structure of an enzyme is destroyed, does an enzyme denature or does denaturation specifically concern the destruction of the tertiary structure?
2) Concerning photosynthesis and cellular respiration, do we ACTUALLY only need to know the outputs and inputs? Teachers always tell us this, but that seems too simple. Do we at least need to know the exact reaction that results in, for example, the oxidation of ATP into ADP? So for glycolysis, the expenditure of ATP occurs during step 1 and step 3. Is that complex enough, or do I need to know the specific names of the enzymes?
3) ATP synthase harnesses the energy from the proton gradient to catalyse the anabolism of ATP from ADP and Pi. HOWEVER, I read somewhere that free e- are also used to help 'fuel' ATP synthase, so to speak. Is this true?
Thank you!!
1. As mentioned earlier on, the quaternary does indeed effect enzyme functioning. If the enzyme is disrupted on this level, the enzyme is denatured as it's specific 3D shape will have changed. Therefore, it won't be able to bind to its specific substrate to catalyse reactions.
2. It is true in the sense that we only need to know the inputs and outputs for each section. However, VCAA can test on the knowledge of whether a specific reaction is endergonic or exergonic, catabolic or anabolic. Also, it won't hurt to have a general understanding of what is happening, and where. You must definitely don't know to know the specific names of enzymes , or proteins involved.
3. Doesn't hydrogen ions move from the matrix into the inner membrane space, and then comes back in via ATP Synthase along the cristae and combines with ADP + Pi to form ATP?