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April 28, 2024, 03:27:08 am

Author Topic: how to work out the amount of dna after pcr?  (Read 579 times)  Share 

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Genshai

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how to work out the amount of dna after pcr?
« on: October 28, 2019, 02:23:10 pm »
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hi all,
i feel like every pcr question is a gamble for me. I thought dna doubled after every pcr, but this doesnt seem to be the answer. is there a formula to figure our how many dna fragments are created after so many cycles of pcr?

please help !!

thank you !!!!
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Evolio

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Re: how to work out the amount of dna after pcr?
« Reply #1 on: October 28, 2019, 03:31:36 pm »
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Hi!
It's usually 2 to the power of n where n is the number of cycles.
This gives you the total amount of DNA produced.