Second Unit 3 SAC HELP!

[Cammmeron!]

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[Yacoubb]

Hey guys, hope your all doing well.

Anyways I just did the practical portion of my second unit 3 sac on enzymes, and I'm having so much trouble thinking up a hypothesis. What we did was test enzyme activity in different solutions that had different pH levels. To test if there was a reaction, we looked at the formation of bubbles, and if a lit splint would ignite when placed in the test tube which indicated the presence of oxygen.

Can someone help me write up the hypothesis?

Many thanks in advance.

Could you give the aim of the experiment + the name of the enzyme?

[Yacoubb]

You need to provide more info about variables!

[Yacoubb]

Okay

There were 5 test tubes, each had a different acidic solution in it. In each test tube, we
placed a piece of liver in each solution and let it sit for 30 mins. After that, I added hydrogen peroxide to each test tube and observed the formation of bubbles. Then I lit a splint and Placed it in the test tube to see if it would ignite to indicate the presence of oxygen.  The solutions we used were acetic acid, sodium chloride, sodium carbonate, hydrogen chloride and distilled water.

There was a 6th test tube that contained a piece of
Liver in distiled water. That was the control. There was no observable formation of bubbles
or reaction because the splint didn't re-ignite.

I know we were testing the the effect of pH on enzyme activity in the liver, but I just can't write a decent hypothesis.

The test tube that produced the highest oxygen volume, as demonstrates by ignition of a lit splint, would be the optimum pH of catalase, whereas the test tube containing catalase with the lowest oxygen volume, would be of a pH either too high or too low that is beyond the optimum pH of the catalae enzyme.

^someone clarify this, but I think its good because:
(A) Introducing IV and DV
(B) Making hypothetical statement

[Yacoubb]

Thanks Yacoub. I'm just wondering, is there any way to reference the use of liver and the different type of acidic solutions and how their range in pH can affect the enzyme. So, for example, say that liver placed in highly acidic solutions become denatured as a result of the altered shape of the active site?

Yep but you did the experiment so find a way to integrate your aim into the hypothesis

What is pH optimum of catalase?