I've been going over some things for the first bio prac and wondering how to figure out the size of the cell. I know that we divide the field of view by how many cells we think fit in that field of view, but I went on the internet, and they were talking about using rulers and what not.
Also, what is the best lens to be used if I wanted to estimate the size of a blood cell? In particular in relation to this question - Calculate the diameter of a red blood cell. which lens is most suitable for doing this?
Hint: get number for 1/4 of the total diameter of field of view, then divide it into 1/4 of the diameter of view.
If it want for the hint, i would of just used the field of view under low objective lens and divided that by the number of cells that fit along the diameter. But, the hint confuses me. What do I do?
S = Size of each cell (in micrometers)
D = Diameter of field of view
N = Number of cells observed
S = D/N
Don't worry about the internet, theres obviously many ways/formulas for everything, at UoM we use that formula.
If you want to estimate the size of a red blood cell, the best lens to use would be the high power lens (x400 mag). This is because by previous experience, RBCs are ~7 micrometers big, and so if you use the low power lenses, then you would need to count only a quarter of the resolution, but there could literally be hundreds to count. So for convenience, we use the high power as there are less cells to count.
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