Dialysis

[stonecold]

Hey, could someone please help me out with this.

If you were doing a dialysis experiment, where it is necessary to remove all of a substance from inside of the dialysis tubing (such as for a scientific experiment on a purified large protein molecule) to outside of the tubing, how would you go about this, and what would be different from the normal dialysis experiment where you have passive movement of molecules down a concentration gradient until equilibrium is reached?

I think it is talking about active transport but I'm really unsure how you would simulate this in a experiment.  Then again, I may be wrong all together lol.

Help much appreciated.  Thanks.

[kenhung123]

Kidney and dialysis is unit 1/2 stuff. Not in the 3/4 course.

[simonhu81292]

a bit was mentioned... but it wasn't that important  :smiley6600:

[herzy]

im actually not sure either, doesnt really sound like what we covered in biol... where'd you find the question?

[stonecold]

it's on my stupid experiment sheet which is assessed.  yeah we did the basic experiment where you have the cellulose tubing, fill it with solution containing glucose, starch, salt and protein, and place it in a beaker.  at the end only the salt and glucose diffuse through.

if you made the pressure higher in the tubing than in the beaker, then that would force ALL of the molecules that would fit the membrane right?  (all of the glucose and salt would get out because of the increased pressure, the flow is only in one direction)

does that sound right/feasible?

[herzy]

sure i guess, but that'd force everything through - its kinda cheating.
maybe you could try continually diluting the external environment or otherwise removing the substance in question, so that the concentration gradient remains in favour of the molecule moving towards the outside...

[stonecold]

^haha!  that sounds wayy better.  thankyou so much.  

i should have thought of that, so simple, damn!  just keep the concentration gradient high.

[herzy]

yepppp technically you'll never get it ALL out but pretty damn close

[slothpomba]

I think he means dialysis tubing not like kidney dialysis maybe if you hugely bumped up the solute concentration or something you'd get a huge chunk out.

[We've actually done a similar SAC with dialysis tubing]