MelbUni Biol SAC

[ninwa]

hi, is anybody going to melb uni on Monday to do gel electrophoresis/ genetic engineering workshop?

[KeyMan]

My class did the same thing (electrophoresis and genetic engineering) yesterday. It wasn't at Melb Uni though, we did it at CSIRO near the Botanical Gardens. The genetic engineering was fun because you get to mutate E.coli into an ampicillin resistant strain on E.coli (assuming you do the same thing my class did).

[melaniej]

We're doing it at Melb uni on wed afternoon!

[Collin Li]

Oh, you can tell me where the Engineering Workshop is then?! One of my tutorials might be there...

[morrjs]

Ahhh u guys are so lucky….we were going to do it at school coz we have the equipment but our teacher lost the gel plates….so he just made up the results for us. It was so boring to write a report after not doing the actual prac

[NE2000]

Hey in gel electrophoresis what's the role of the buffer?

[bturville]

so that it can conduct electricity.

[NE2000]

Does it control ionization of acids in the gel? I mean does it make the gel conduct electricity by maintaining the pH at a certain level?

[NE2000]

And also if anyone did an electrophoresis experiment, are there any systematic errors you could identify?