CAN SOMEONE PLEASE EXPLAIN TO ME THE WHOLE GENE EXPRESSION/REGULATION STUFF...GOING THROUGH IT NOW AND I'M A TAD CONFUSED....WHAT IS THIS WHOLE LAC OPERON STUFF??
Transcript:
- RNA polymerase transcribes the DNA template strand.
- pre-mRNA is synthesised 5' to 3' by complementary base pairing.
- pre-mRNA undergoes post-transcriptional modification (includes intron removal).
- mRNA leaves the nucleus & enters the cytoplasm via nuclear pores.
Translation:
- mRNA is read by the ribosome.
- When an mRNA codon is read, the anticodon of a loaded tRNA molecule binds to the mRNA codon by complementary base pairing.
- The loaded tRNA then deposits the amino acid, which is added to the growing polypeptide chain.
- A polypeptide is synthesised.
^^ Thats all you need!
The lac operon is found in bacteria. It shows how gene regulation occurs in prokaryotes. In the absence of lactose, a repressor protein binds to the promotor, preventing RNA polymerase from binding to the promotor, thus preventing the transcription of the gene for lactase.
However, when lactose is present, lactose binds to & induces a change in the repressor protein. The promotor is unoccupied, RNA polymerase can bind to the promotor, and transcription of the lactase gene can occur.
Remember that gene regulation is needed to conserve energy, and produce proteins only when and where they are required.