Genes & Genomes exam thread

[jinny1]

I got an email from John saying

Please be aware that the GENE20002 course content changed this year. This means there will be some exam questions from previous years that you may not be able to answer. If you have no idea what the question is asking, DON'T PANIC, it is likely that this question covers material that is no longer taught.

[Starlight]

2008.

Q28: C

I agree it is C, lecture 33 slide 9 basically gives you the answer.

Q32: I have no freaking idea... Did we go though this in our lectures??? :S

2010 exam

Q28: A

Yea i remember reading that to increase copy number, reduce size of the plasmid.

Q31: D

I'm not sure about this either!! Wow the questions you've presented so far are pretty hard.....I'm hoping you only picked out the hardest ones I havne't looked at the whole exam yet.

Maybe Golden can help.

LOL btw i can't find the 1998 exam on the LMS Will have to search for it later somehow.

But for now, i'm actually enjoying memorising the step of glycolysis!! IT's surprisingly fun haha

The 1998 exam is on the library past exams website But thanks for helping me out with the other questions!

[Starlight]

2010- Q 34, do you guys think D is incorrect? The other three I think he has mentioned in lectures but not sure about the 4th

[jinny1]

2010- Q 34, do you guys think D is incorrect? The other three I think he has mentioned in lectures but not sure about the 4th

err they all seem pretty correct to me

 Any particular reason you think D is wrong???

I'll ask the lecturer.

[Starlight]

err they all seem pretty correct to me

 Any particular reason you think D is wrong???

I'll ask the lecturer.

It's a tough question that's for sure. These are my thoughts:
I think A is good because that describes protein recognition, one of the suggested approaches.

I'm tossing up between B and C as the approaches you would not use.

 I think D is correct because it describes using functional complementation and the approach looks like it is being done properly, i.e. isolating the his auxotrophs which have now been turned into a his prototrophs.

The reason I thought B was because it says a "degenerate oligonucleotide", however in the lectures we have been told that the olignonucleotide probe should be designed from the least degenerate region of the mrna sequence.
The reason I thought C might be incorrect is because I think the library should be made from the His6 wild-type strain.

So would you say B or C? :S I'm leaning towards C

q34 from 2008 has a similar question

[Starlight]

Also for 36 do you think a?

[Frost]

I think I'm missing the slides for lectures 20 and 21, could someone upload them or pm them to me please?

[Starlight]

^ Pretty sure they're off the LMS.

[Starlight]

btw jinny1, looks like it might be just me and you discussing exam qs, golden hasn't been on for a while...

[jinny1]

It's a tough question that's for sure. These are my thoughts:
I think A is good because that describes protein recognition, one of the suggested approaches.

I'm tossing up between B and C as the approaches you would not use.

 I think D is correct because it describes using functional complementation and the approach looks like it is being done properly, i.e. isolating the his auxotrophs which have now been turned into a his prototrophs.

The reason I thought B was because it says a "degenerate oligonucleotide", however in the lectures we have been told that the olignonucleotide probe should be designed from the least degenerate region of the mrna sequence.
The reason I thought C might be incorrect is because I think the library should be made from the His6 wild-type strain.

So would you say B or C? :S I'm leaning towards C

q34 from 2008 has a similar question

hmmm i think you are correct about B!

How can there be functional complementation if they are using a His6 mutant cDNA library to test for complementation???

Wouldn't they have to use a His6+ cDNA library??? So yea i think you are right about B. I would've never figured it out.

btw jinny1, looks like it might be just me and you discussing exam qs, golden hasn't been on for a while...

that sucks he is supposed to be the smart one :/

[jinny1]

Also for 36 do you think a?

I'm leaning towards D...

A sounds good but the keyword is "rapid". Wouldn't northern blot take longer because they have to test multiple RNA samples from varying developmental stage, environmental conditions etc?? Because the RNA expressed always varies but DNA is always the same.

[Starlight]

I'm leaning towards D...

A sounds good but the keyword is "rapid". Wouldn't northern blot take longer because they have to test multiple RNA samples from varying developmental stage, environmental conditions etc?? Because the RNA expressed always varies but DNA is always the same.

Just clarifying with 2010 q 34. Did we decide that C was the approach we wouldn't use i.e. the correct answer? Because you can't select the clones that can now grow on histidine as the auxotrophs are just being inserted with genetic material that doesn't allow them to synthesize histidine?

With 36.. just eliminating some options:

b and c implying the same thing- just looking for proteins but won't be of help as all four genomic clones probably produce proteins

I was thinking it wouldn't be d because it says "plant genes" whilst a mentions "flowers" which is what the arabidopsis thing is. Like it seems a is more specific in its action?

[jinny1]

Just clarifying with 2010 q 34. Did we decide that C was the approach we wouldn't use i.e. the correct answer? Because you can't select the clones that can now grow on histidine as the auxotrophs are just being inserted with genetic material that doesn't allow them to synthesize histidine?

Yea sorry i meant C was the incorrect answer (and hence the correct answer that you would circle). You would use B but not C. I got confused with the letters.

I was thinking it wouldn't be d because it says "plant genes" whilst a mentions "flowers" which is what the arabidopsis thing is. Like it seems a is more specific in its action?

oh true. freaking hell i never pick up on these minor points.

I think you are correct in saying the answer is A for Q36. Because we are looking for a gene specifically in arabidopsis not any other plant.

[Starlight]

Yea sorry i meant C was the incorrect answer (and hence the correct answer that you would circle). You would use B but not C. I got confused with the letters.


oh true. freaking hell i never pick up on these minor points.

I think you are correct in saying the answer is A for Q36. Because we are looking for a gene specifically in arabidopsis not any other plant.

Haha don't worry I mainly pick them up after reading a couple of times. But it SEEMS as though most the time the answer with "northern blot" is the correct answer, but just make sure to bring your highlighter in reading all the different answers

[Starlight]

2010: Q 3 would you say a?
and for Q 9 b or a?
and did we even learn about 19?