Hey,
For this question (Qu25 MC from 2017 NHT) the answer is D, but I choose B. Can someone explain this please.
My line of thinking was:
1. Complementary sequence: opposite of given DNA sequence
2. mRNA of complementary sequence would be the opposite of the complementary
(the same as DNA sequence except for the mutation and T is replaced with U)?
Thank you!
The strand they have given you is the template strand, but mRNA makes a complementary copy to the template strand, swapping T with U (so its sequence of bases is identical to the coding strand). This means mRNA will carry the complementary copy of the DNA sequence given, making D the correct answer. I think you've gotten a little confused with the transcription process - if you go back and revise the steps from your textbook/notes it'll make sense.
1. DNA unwinds to expose the template strand
2. RNA polymerase attaches to a promotor in the upstream flanking region of the gene
3. RNA Polymerase catalyses joining of RNA nucleotides that are complementary to DNA template
4. Template strand of DNA is copied (transcribed) to form a pre-mRNA strand (complementary copy), primary mRNA transcript has
been formed
These are the first few steps of transcription, and as you can see mRNA forms a copy that is complementary to the sequence on the template strand, which is the strand we start off with.