when doing gel electrophoresis, does every band have to match up exactly for two people to be related. Also how do determine the distance between two bands as often it relies on guessing.
Well it would be expected that the bands would align pretty much perfectly if the individuals have the
same alleles, this is because the restriction enzyme cuts at a specific recognition site. thus because the same alleles have the same DNA base sequence the restriction should cut the DNA sequence into equal strands with equal number of kilobases, so they move through the agar gel at the same speed.
It would be safe to assume if they wanted you to measure the distance between the two bands, the examiners would provide a standard which contains bands of known length and how far they travel through the gel during the process of electrophoresis. otherwise it would be pretty dodgy