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June 28, 2025, 07:54:17 pm
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Author Topic: Rishi's Biology Thread  (Read 24386 times)  Share 

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Rishi97

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Re: Rishi's Biology Thread
« Reply #60 on: July 28, 2014, 09:07:09 pm »
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What is the difference between PCR and gene cloning?
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Reus

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Re: Rishi's Biology Thread
« Reply #61 on: July 28, 2014, 09:10:13 pm »
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Quote from: Rishi97 on July 28, 2014, 09:07:09 pm
What is the difference between PCR and gene cloning?
This site has on point information, helped me so it may help you! :)
http://www.majordifferences.com/2013/10/difference-between-gene-cloning-and-pcr.html#.U9YvXV7vbfM
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Rishi97

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Re: Rishi's Biology Thread
« Reply #62 on: July 28, 2014, 09:34:24 pm »
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Quote from: Reus on July 28, 2014, 09:10:13 pm
This site has on point information, helped me so it may help you! :)
http://www.majordifferences.com/2013/10/difference-between-gene-cloning-and-pcr.html#.U9YvXV7vbfM

Perfect!!! Just the areas that I needed clarification on
Thanks Reus :)
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Rishi97

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Re: Rishi's Biology Thread
« Reply #63 on: July 29, 2014, 05:13:39 pm »
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Would I be correct to say that primers "instruct" the DNA polymerase to start replicating the DNA strand in PCR?
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nerdmmb

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Re: Rishi's Biology Thread
« Reply #64 on: July 29, 2014, 05:20:07 pm »
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Quote from: Rishi97 on July 29, 2014, 05:13:39 pm
Would I be correct to say that primers "instruct" the DNA polymerase to start replicating the DNA strand in PCR?

I don't think they instruct..

If I'm not mistaken, they just act as a starting point for the attachment of DNA/RNA polymerase.

:)
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vox nihili

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Re: Rishi's Biology Thread
« Reply #65 on: July 29, 2014, 05:58:50 pm »
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Quote from: Rishi97 on July 29, 2014, 05:13:39 pm
Would I be correct to say that primers "instruct" the DNA polymerase to start replicating the DNA strand in PCR?

They give you the replication fork, which as nerdmmb said, is where DNA pol can attach
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Rishi97

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Re: Rishi's Biology Thread
« Reply #66 on: August 11, 2014, 05:32:36 pm »
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The human growth hormone consists of 14 amino acids. The minimum number of codons for this hormone would be:
a) 14
b) 16
c) 42
d) 48

Thanks  :D
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Reus

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Re: Rishi's Biology Thread
« Reply #67 on: August 11, 2014, 05:44:24 pm »
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Quote from: Rishi97 on August 11, 2014, 05:32:36 pm
The human growth hormone consists of 14 amino acids. The minimum number of codons for this hormone would be:
a) 14
b) 16
c) 42
d) 48

Thanks  :D
Don't three amino acids make one codon?
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Rishi97

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Re: Rishi's Biology Thread
« Reply #68 on: August 11, 2014, 05:46:30 pm »
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Quote from: Reus on August 11, 2014, 05:44:24 pm
Don't three amino acids make one codon?

I thought it was 3 nucleotides make up one codon which codes for one amino acid.
Not sure though
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vox nihili

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Re: Rishi's Biology Thread
« Reply #69 on: August 11, 2014, 06:35:53 pm »
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Quote from: Rishi97 on August 11, 2014, 05:46:30 pm
I thought it was 3 nucleotides make up one codon which codes for one amino acid.
Not sure though

You're right
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Reus

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Re: Rishi's Biology Thread
« Reply #70 on: August 11, 2014, 06:47:46 pm »
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Quote from: Rishi97 on August 11, 2014, 05:46:30 pm
I thought it was 3 nucleotides make up one codon which codes for one amino acid.
Not sure though
Haha sorry, got them mixed up! Yep, they do. A codon codes for one amino acid.
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Rishi97

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Re: Rishi's Biology Thread
« Reply #71 on: August 11, 2014, 07:00:43 pm »
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Quote from: Reus on August 11, 2014, 06:47:46 pm
Haha sorry, got them mixed up! Yep, they do. A codon codes for one amino acid.

ok so do you know how to get the answer?
I did 14 x 3 and so circled 42 but my teacher said that the correct answer is 16! How?
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grannysmith

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Re: Rishi's Biology Thread
« Reply #72 on: August 11, 2014, 07:13:43 pm »
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Should be 42..

Edit: my bad, got it mixed up. It's probably 16. So each amino acid is coded by one codon. But you have to take into account stop and start codons. therefore 14 + 2 = 16.
« Last Edit: August 11, 2014, 08:05:20 pm by grannysmith »
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Rishi97

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Re: Rishi's Biology Thread
« Reply #73 on: August 11, 2014, 07:15:58 pm »
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Quote from: grannysmith on August 11, 2014, 07:13:43 pm
Should be 42..

Yeah thought so... i'll ask him tomorrow and hopefully grab that extra mark :P
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Paulrus

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Re: Rishi's Biology Thread
« Reply #74 on: August 11, 2014, 07:47:05 pm »
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Quote from: Rishi97 on August 11, 2014, 07:15:58 pm
Yeah thought so... i'll ask him tomorrow and hopefully grab that extra mark :P
your teacher is right i think. the questions asks how many codons there are, which are the base triplets codes. so if there are 14 amino acids, each will have one codon which is made up of three base pairs.
then in your sequence you need the start and stop codons as well, so that makes 16 codons total.
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