There was a question regarding the steps and procedures of PCR (Polymerase Chain Reaction). It was a 3 mark question so I assume there are 3 parts to it. I listed each step- explaining what occured at each step including the appropriate temperatures at which the different events occured. However, I am worried that I forgot to mention that the process should be repeated at the end. Do you think I will lose a whole mark for this? Technically speaking though, the question asked how scientists may amplify samples of DNA and even one cycle of PCR would produce 2 new strands of DNA, technically 'amplifying' the original amount... So my question is, do you think I will lose an entire mark although I listed all 3 steps and all important factors correctly?
This question has been asked before and here was VCAA's answer:
heat DNA to approximately 90 °C or to separate strands
cool to approximately 50 °C or to anneal/attach primers
heat to approx 72 °C or Taq/DNA polymerase copies strands
repeat cycle.
Many students set out clear answers that demonstrated a sound understanding of the process. Many other students
missed out on full marks by not stating that the process needed to be repeated.
Although, this question was out of 4 marks and the one on our exam was maybe out of 3 marks?
But yeah might lose the mark.. but hopefully not because i think i forgot to say repeat cycle as well
