Hii, I was wondering if someone could clarify this Genetics for point as I find it really confusing: ‘Explain how the use of recombinant DNA technology can identify the position of a gene on a chromosome.’
My understanding is this:
1. A restriction enzyme is used to cut a section of DNA
2. The fragments are placed on gel where electrical impulses cause fragments to separate by size (gel electrophoresis).
3. The fragments are then stained by fluorescent dye which glows, and cut again.
4. A radioactive probe (single stranded sequence of DNA that is complementary to the gene) is are tagged with radioactive marker, and attached to complementary gene and inserted into human chromosome.
5. When a light is shone, the probe glows showing the location of the gene.
That’s what I recall from reading notes, however I don’t understand how the process (if that’s even correct) is recombinant DNA technology or why original DNA is cut up TWICE and probe isn’t just added in initially ugh smh.
Any help would be greatly appreciated

(.