HAVE YOU GUYS LEARNED CLONING TECHS? WTFF?

[matt123]

lol guys

I just did insight 07 paper.

wtf is nuclear transfer?
ive never heard of these techniques.

have you guys learned them?

[itsMRPHI]

lol we had to study that on our own. that shit better not turn up on the exam

[Keine]

I did that and there were many ambiguity which
annoyed me.. a lot :\
Other than that, I had no idea what the hell 'nuclear transfer'
was and frankly, I recall not ever reading about it
This better not come up on the exam!!

[yuuhei]

what the...
some kind of way to transfer a gene to another organism? to clone? Or something??? o.o

[slothpomba]

Doesn't really ring a bell either... but sometimes obscure shit you dont learn comes up on the exam, there was a question on "fractioning" a couple years back, it isnt in nature of biology, we didnt learn it but it was on the exam (it was however in that biology activity book thingy forget the name...biozone?)

Just hope it doesnt show up

[masonnnn]

i was thinking a very similar thing. Our final sac was based around dna technologies like cloning and stem cell research, yet it annoyed me as looking at exams there is never any questions on it, so seemed a waste of time.
though since then i have noticed a few questions, if you have the most basic understanding of nuclear transfer that's the most i've seen required. all questions relating to it seem to usually provide the example and the answer can be determined from interpreting info. in the question correctly.

one of the huge things we've noticed with bio is that S.A isn't usually a lot of definition questions so much as interpretation AND those questions that basically have the answers within themselves yet require interpretation tend to be those that 80% of people answer incorrectly.

[The Detective]

came across this question too and got it wrong, nope haven't learnt it

[cypriottiger]

luckily i did it as my sac for gene technology so if you have any questions specifically let me know and hopefully i can answer it for you

[The Detective]

luckily i did it as my sac for gene technology so if you have any questions specifically let me know and hopefully i can answer it for you

thanks cypriottiger, so what is nuclear transfer?

[cypriottiger]

well its relatively simple to understand
cloning involves taking the nucleus of the desired organism to be cloned, and implanting it into the egg cell of the surrogate.
so the egg cell from the surrogate animal is obtained, and a microscopic vacuum is used to 'suck out' the nucleus, leaving only a membrane and cytoplasm. this is done to ensure that there will be a high probability that the cell will be reaccepted by the 'mother', as it is not foreign.
the desired organism has its nucleus carefully removed, and remaining cell is discarded. in brief the 'mother's' cell is deprived of nutrients to make it more susceptible to accepting foreign material.
the nucleus and mother egg cell are held together and a small electric current is applied, allowing for the nucleus to be accepted. this is nuclear transfer.
i guess all thats left in cloning is for the complete cell to be allowed to grow for several mitotic divisions in a laboratory before implantation into the original 'mother' organism

[golden]

I also want to know how much we have to know - there are a few processes of cloning.
Nuclear transfer, embryo splitting, etc.

Should we be able to know each step of some methods of cloning?

[herzy]

the knowledge cypriottiger gave should suffice (ie a general overview), but you DO NEED TO KNOW IT
if you have nature of biology, it's in there and you should read it. otherwise know nuclear transfer (where they transfer the nucleus of a somatic cell into an egg or something) and all about gene therapy, vectors, transformation (bacteria, bacteriophages,) etc etc

[cypriottiger]

I also want to know how much we have to know - there are a few processes of cloning.
Nuclear transfer, embryo splitting, etc.

Should we be able to know each step of some methods of cloning?

um embyro splitting im not really aware of......
the main differences you need to be aware of are in therapeutic cloning and embryonic cloning.
therapeutic cloning involves (though im not 100% sure) taking the embryonic technique further. a stem cell is allowed to undergo mitotic divisions in a laboratory, effectively growing the tissue it was predisposed to be. im not really sure on this area though, but i think you just need to be aware of therapeutic cloning's use as an alternative to gene therapy, although they are very similar.

[masonnnn]

douchy's episode on gene technologies is good for this. he summarizes gene therapy towards the end.
and aslong as your understand the terms and general idea of cloning that'd be enough.

[herzy]

therapeutic cloning is important. embryonic splitting is when an embryo (blastocyte) is dividing, and you keep splitting it, so it keeps dividing (kinda like identical twins)... if you understand genes, and cells, you'll be fine.