You really are just setting up a point of comparison so that any effects on the activity of amylase can be attributed to the independent variable, which in this case, is temperature. You are ultimately comparing the rate of enzymatic activity in different temperature conditions to determine temperature optima, and so its imperative you have a point of comparison at which you have variables controlled like temperature; if you didn't have it at room temperature, than we can say that enzymatic activity at 30 degrees C would not be as efficient as 20 degrees C, because room temperature may have had some influence. Its eliminating all those extraneous variables and attributing all effects on the DV with the IV under investigation.