On monday I had my sac on enzymes, and was wondering if anyone has done it and what type of questions do I prepare myself for?
I don't particularly understand why liver was used? We cooked one to test the rate of reactions? But we also recorded the different colours we saw after we mixed amylase with iodine, at different temperatures?
Can someone kindly give me a run down of the things I should study, and the things I should know?
thank you for anyones help!
I am not so sure about the liver. But the temperature one and amylase was identical to mine.
We had to test 4 tests in differing temperatures where we observed the performance of amylase under those conditions. Basically, if there was no starch digestion (break down), it meant the the amylase had completely denatured when it was exposed to the extreme temperature. The other one was freezing temp, where the digestion of starch was there, but it was at a slow rate, meaning that the enzyme was exposed to low temperatures but was still able to function. The final test was at normal room temperature. The enzyme worked at it's optimal, as the starch was digested relatively faster.
Some questions on the SAC included:
1. Enzymes are biological catalysts, explain
2. What is the independent variable of the experiment?
3. What is the dependent variable of the experiment?
4. Explain the experimental results and what is the enzyme's optimum temperature?
5. What was expected (in terms of enzymatic activity) when amylase was placed in : 0-10C, 20-30C and 80-100C?