what did you guys write for the YAC and why the centromere?
Ahahahaha
ahahahaha
I was like UMMMMMMMM.
I put something about it being there to hold the allelic DNA and the whatever the other thing was in place because that chromosome was a free and isolated one, whereas the plasmid didn't need one because it's circular and therefore all DNA will be held in place without a centromere.
Yeah. I had no idea.
If I understand this question correctly, what others have said regarding spindle fibres is correct. In prometaphase (midway between prophase and metaphase), the spindle fibres from the centromeres attach to proteins located on either side of the centromere, and in anaphase after the centromere is cleaved, these fibres then separate the two sister chromatids. This isn't required in bacteria because their DNA reproduces by binary fission instead of mitosis.