The Wagyu CHSI allele was isolated and given a fluorescent tag. it was introduced into a yeast cell as a large, independent, cytoplasmic chromosomal segment called a yeast artificial chromosome (YAC). in addition to the allelic DNA, a YAC includes a centromere and a replication sequence. the yeast cells are then incubated in the presence of growth stimulants and given time to replicate. this procedure is similar to genetic engineering of bacterial plasmids, however the YAC is able to contain much larger pieces of DNA than a plasmid.
cii bacterial plasmids lack a centromere. why are yacs made with a centromere
iii. what term describes the process of copying a gene
c.ii. Yeasts are eukaryotic organisms and hence duplicate DNA via mitosis, which requires the centromere as an anchor point for spindle fibres during mitosis. In contrast, bacteria duplicate DNA via binary fission which does not require spindle fibres.
If this was a one marker, simply stating 'to allow replication' MIGHT be enough.
c.iii. Under the scenario, I'm inclined to say cloning as the emphasis of the scenario is genetic engineering as stated. If they wanted to test DNA replication, they'd ask specifically for it. But as I said previously, depends on leniency of VCAA markers.