hiii its me again... low-key stressing over here 😅but for 10b) I didn't really know what to put down so I wrote smth along the lines of how the insulin protein structure and amino acid chain are needed to be known as they are important for the correct functioning of the insulin protein and so if the insulin gene in the plasmid produced insulin protein that had an amino acid chain and a protein shape different from that which would be coded for by an normal human insulin gene, the function would be impaired or it wouldn't function properly. would this be ok cuz I'm not very sure??
also for my 11d) I said that the results of the experiment did support the predicted -( I described how they had the same overall trend) like on Day 0 in both predicted and experiment results, there was more bacteria growing on the plate of the control group compared to the experimental group and on the final day (Day 3) I said that there was less bacteria growing on the plate of the control group and more bacteria growing on the plate of the experimental group. would this still be okay bc I'm not too sure??
Not really, so the idea was that if we knew the amino acid sequence, then we could artifically create that DNA sequence to be inserted into the plasmid and because we're basing it on the amino acid sequence, there won't be any introns. Another way to think about the latter point is the idea that bacteria won't be able to undergo post-transcriptional modifications, so it makes sense that there won't be any introns.
Yeh, I can see where you're coming from. From my quick glance it didn't really look like it matched up completely, but maybe you'll be able to argue that the trends are similar.
With the recombinant plasmid q it asked about how the gene would be cloned and the gene would be expressed in the bacteria. U spoke about restriction enzyme and all that which is to do with uptake and formation of the plasmid. I spoke about how it would be left to replicate on the nutrient agar plate after they were exposed to an antibiotic such as ampicillin and those not transformed would die and transformed would survive. Then the scientists would induce these transformed bacteria to express the human insulin gene. I might be wrong but that’s how I interpreted the q.
Yep, I can definitely see where you're coming from, and I guess its just come in at the difference in interpretation. So the question was outline the steps that are required for the human insulin gene to be cloned and expressed in bacteria - the way I read this is how do we clone an insulin gene? and how do we express it in bacteria? We clone it through the use of plasmids, and we express it through the method of recombinant plasmids.
Hi, do you remember what the wording of the Octopus question part b was? I interpreted it as lower genetic diversity in the NZ population so I said that genetic bottlenecks from heavy predation or the founder effect acted, but now I think that's wrong based on you saying gene flow occurred. would you be able to explain this question please?
Also do you think a score of 115/120 would get a raw 50?
This question was talking about the lack of genetic diversity between the two populations - meaning the two populations are similar genetically. The idea of gene flow is that if we're having gene flow between the two populations, then differences cannot accumulate. Study score depends on how the overall state does on the exam, but if for example it was the 2018 exam, a 115/120 with high A+ SACs would definitely be a raw 50.
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Topic: VCE Biology 2021 Exam Solutions (Read 23448 times)