Transcription and Translation

[lozie.1]

Need help in fully explaining he process, not exactly clear on it!

[yuuhei]

Well.. I will try and explain and people correct me if I'm wrong or missing anything. Also I hope this addresses your question.  

Transcription is pretty much obtaining the mRNA from DNA. Process starts at the nucleus where the DNA is unwind and the RNA polymerase creates it.. much like DNA replication but with RNA polymerase instead of DNA polyermase and Uracil instead of Thymine.

I think there's an extra step where after this results in pre mRNA formed and the pre mRNA is cut/spliced into pieces. All of the introns (bits not necessary for the target protein) are thrown out and the extrons are combined making that final mRNA.

Translation is making the protein or whatever that the mRNA codes for. Process starts at cytoplasm where the mRNA is escaped from the pores of the nucleus.

The mRNA goes to the rRNA and the tRNA reads the codon (by 3 bases eg AUG).

Each tRNA are different and have specific anti-codons to match the complementary codons on the mRNA along with it's animo acid attached.

So from say the tRNA with AUG.. looking at the table (will be provided) it will drop off a Met animo acid and the next 3 bases will be read by another tRNA and so on. That tRNA (the one that dropped off Met) will be reloaded/recharged getting another Met animo acid. And... you should know that the animo acids joined together by peptide bonds.

Finally, after this has reached the stop codons putting an end of translation, the animo acids eventually become a polypeptide and go through that protein formation of folding up and ect...

[Russ]

Couple of clarifications

I think there's an extra step where after this results in pre mRNA formed and the pre mRNA is cut/spliced into pieces. All of the introns (useless bits that don't code for anything) are thrown out and the extrons are combined making that final mRNA with the help of DNA ligase.

Don't call introns useless bits, some of them are important in coding for other proteins. Say they're not necessary for the target protein. Also, DNA ligase is involved in transcription?

[yuuhei]

thanks for the clarifcation for introns!
Umm.. ok.. nevermind about DNA ligase.. lol
I think I was thinking of something else xD

[masonnnn]

so easy to get transcription and translation mixed up too and stuff up the easy 1 mark questions.