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August 24, 2025, 09:32:50 am
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Author Topic: PCR  (Read 1176 times)  Share 

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itsMRPHI

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PCR
« on: October 28, 2010, 08:09:39 pm »
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Do nucleotides get added from the 5' to 3' direction or the 3' to 5' direction?
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jasoN-

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Re: PCR
« Reply #1 on: October 28, 2010, 08:10:34 pm »
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Russ

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Re: PCR
« Reply #2 on: October 28, 2010, 08:12:32 pm »
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Erm, what?
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TrueLight

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Re: PCR
« Reply #3 on: October 28, 2010, 08:17:22 pm »
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shinny

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Re: PCR
« Reply #4 on: October 28, 2010, 08:25:56 pm »
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The enzymes read/act in a 3' to 5' direction, meaning the nucleotides are added in the 5' to 3' direction with respect to the complementary strand.
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masonnnn

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Re: PCR
« Reply #5 on: October 28, 2010, 08:56:11 pm »
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Quote from: shinny on October 28, 2010, 08:25:56 pm
The enzymes read/act in a 3' to 5' direction, meaning the nucleotides are added in the 5' to 3' direction with respect to the complementary strand.

This.
Just know that no matter what they are always ADDED to the 3' end.
this gets confusing as because they are added to the 3' end the template is actually read in a 3'-->5' direction.
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itsMRPHI

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Re: PCR
« Reply #6 on: October 28, 2010, 10:36:23 pm »
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cheers mason
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