Is it correct to say the Second Line of Defence involves recognising 'self' from 'non-self'? Or is that in the 3rd line?
The second line of defence involves macrophages recognising non-self (foreign) matter, and then binding to, engulfing & eventually destroying this antigen. However, the macrophages do not distinguish between different pathogens in the 2nd line of defence. Instead, they initiate the same response to any type of antigen that they come across, disregarding how often the immune system has come into contact with this pathogen, because there is no immunological retention of memory involved.
So in a sentence, you can say that the second line has self-non self recognition; however, it is the 3rd (adaptive) line of defence that initiates specific responses when exposed to specific pathogens, possessing specific antigenic markers